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KMID : 0385520080210020102
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Analytical Science & Technology
2008 Volume.21 No. 2 p.102 ~ p.108
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Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP-HPLC
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Tian Minglei
Yan Hong-Yuan
Row Kyung-Ho
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Abstract
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Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 ㎎/mL and 1.0 ㎎/mL for LQ and GA, and 0.01 ㎎/mL-0.2 ㎎/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
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KEYWORD
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liquiritin, glycyrrhizic acid, glabridin, licorice, RP-HPLC
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